| Filename | Total | Flagged As Poor Quality | Sequence Length | %GC |
|---|---|---|---|---|
| 01-transhet-1_S1_merged_R1_001.fastq.gz_T1.fastq.gz | 30,626,169 | 0 | 6-76 | 42 |
| 02-V1482Afs-4_S2_merged_R1_001.fastq.gz_T1.fastq.gz | 36,193,128 | 0 | 6-76 | 43 |
| 03-WT-1_S3_merged_R1_001.fastq.gz_T1.fastq.gz | 32,915,659 | 0 | 6-76 | 44 |
| 04-R122Pfs-1_S4_merged_R1_001.fastq.gz_T1.fastq.gz | 33,961,989 | 0 | 6-76 | 43 |
| 05-transhet-4_S5_merged_R1_001.fastq.gz_T1.fastq.gz | 34,729,363 | 0 | 6-76 | 44 |
| 06-transhet-2_S6_merged_R1_001.fastq.gz_T1.fastq.gz | 35,107,542 | 0 | 6-76 | 43 |
| 07-WT-5_S7_merged_R1_001.fastq.gz_T1.fastq.gz | 33,842,919 | 0 | 6-76 | 44 |
| 08-WT-3_S8_merged_R1_001.fastq.gz_T1.fastq.gz | 31,530,778 | 0 | 6-76 | 43 |
| 09-transhet-3_S9_merged_R1_001.fastq.gz_T1.fastq.gz | 32,841,120 | 0 | 6-76 | 44 |
| 10-R122Pfs-4_S10_merged_R1_001.fastq.gz_T1.fastq.gz | 33,256,693 | 0 | 6-76 | 44 |
| 11-transhet-5_S11_merged_R1_001.fastq.gz_T1.fastq.gz | 36,513,992 | 0 | 6-76 | 44 |
| 12-V1482Afs-1_S12_merged_R1_001.fastq.gz_T1.fastq.gz | 37,740,925 | 0 | 6-76 | 44 |
| 13-R122Pfs-6_S13_merged_R1_001.fastq.gz_T1.fastq.gz | 37,194,075 | 0 | 6-76 | 45 |
| 14-V1482Afs-2_S14_merged_R1_001.fastq.gz_T1.fastq.gz | 35,784,157 | 0 | 6-76 | 45 |
| 15-WT-9_S15_merged_R1_001.fastq.gz_T1.fastq.gz | 35,097,070 | 0 | 6-76 | 43 |
| 16-R122Pfs-3_S16_merged_R1_001.fastq.gz_T1.fastq.gz | 34,652,025 | 0 | 6-76 | 43 |
| 17-V1482Afs-5_S17_merged_R1_001.fastq.gz_T1.fastq.gz | 32,997,758 | 0 | 6-76 | 43 |
| 18-R122Pfs-2_S18_merged_R1_001.fastq.gz_T1.fastq.gz | 33,953,908 | 0 | 6-76 | 44 |
| 19-transhet-6_S19_merged_R1_001.fastq.gz_T1.fastq.gz | 33,908,284 | 0 | 7-76 | 44 |
| 20-WT-6_S20_merged_R1_001.fastq.gz_T1.fastq.gz | 37,547,140 | 0 | 7-76 | 45 |
| 21-R122Pfs-5_S21_merged_R1_001.fastq.gz_T1.fastq.gz | 35,000,112 | 0 | 6-76 | 45 |
| 22-V1482Afs-6_S22_merged_R1_001.fastq.gz_T1.fastq.gz | 37,220,154 | 0 | 6-76 | 44 |
| 23-V1482Afs-3_S23_merged_R1_001.fastq.gz_T1.fastq.gz | 34,868,128 | 0 | 6-76 | 44 |
| 24-WT-4_S24_merged_R1_001.fastq.gz_T1.fastq.gz | 37,388,870 | 0 | 6-76 | 44 |
Library Sizes ranged between 30,626,169 and 37,740,925 reads.
Read totals for each library. Duplicated reads are conventionally an high overestimate at this point.
Summary of FastQC flags for each parameter
Heatmap showing mean base qualities for each library
Heatmap showing mean sequence qualities for each library
Heatmap of summed base distributions along each read
GC Content Heatmap normalised to theoretical GC content in the Hsapiens Genome
GC Content Distributions for all reads showing theoretical GC content from the Hsapiens Genome
Universal Adapter Content
Total overrepresented sequences for each library
## Overrepresented_sequences missing from 01-transhet-1_S1_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 02-V1482Afs-4_S2_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 03-WT-1_S3_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 04-R122Pfs-1_S4_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 05-transhet-4_S5_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 06-transhet-2_S6_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 07-WT-5_S7_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 08-WT-3_S8_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 09-transhet-3_S9_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 10-R122Pfs-4_S10_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 11-transhet-5_S11_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 12-V1482Afs-1_S12_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 13-R122Pfs-6_S13_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 14-V1482Afs-2_S14_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 15-WT-9_S15_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 16-R122Pfs-3_S16_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 17-V1482Afs-5_S17_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 18-R122Pfs-2_S18_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 19-transhet-6_S19_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 20-WT-6_S20_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 21-R122Pfs-5_S21_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 22-V1482Afs-6_S22_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 23-V1482Afs-3_S23_merged_R1_001.fastq.gz_T1.fastq.gz## Overrepresented_sequences missing from 24-WT-4_S24_merged_R1_001.fastq.gz_T1.fastq.gz## No overrepresented sequences were detected by FastQCR version 3.6.0 (2019-04-26)
Platform: x86_64-apple-darwin15.6.0 (64-bit)
locale: en_AU.UTF-8||en_AU.UTF-8||en_AU.UTF-8||C||en_AU.UTF-8||en_AU.UTF-8
attached base packages: grid, parallel, stats4, stats, graphics, grDevices, utils, datasets, methods and base
other attached packages: pander(v.0.6.3), ngsReports(v.1.0.1), ggeasy(v.0.1.1), ggrepel(v.0.8.1), pheatmap(v.1.0.12), kableExtra(v.1.1.0), knitr(v.1.26), scales(v.1.1.0), RColorBrewer(v.1.1-2), corrplot(v.0.84), RUVSeq(v.1.18.0), EDASeq(v.2.18.0), ShortRead(v.1.42.0), GenomicAlignments(v.1.20.1), SummarizedExperiment(v.1.14.0), DelayedArray(v.0.10.0), matrixStats(v.0.55.0), Rsamtools(v.2.0.0), Biostrings(v.2.52.0), XVector(v.0.24.0), BiocParallel(v.1.18.0), Biobase(v.2.44.0), fgsea(v.1.10.0), Rcpp(v.1.0.3), rtracklayer(v.1.44.0), AnnotationHub(v.2.16.0), BiocFileCache(v.1.8.0), dbplyr(v.1.4.2), qvalue(v.2.16.0), biomaRt(v.2.40.1), readxl(v.1.3.1), forcats(v.0.4.0), stringr(v.1.4.0), dplyr(v.0.8.3), purrr(v.0.3.3), readr(v.1.3.1), tidyr(v.1.0.0), tibble(v.2.1.3), ggplot2(v.3.2.1), tidyverse(v.1.2.1), reshape2(v.1.4.3), magrittr(v.1.5), GenomicRanges(v.1.36.0), GenomeInfoDb(v.1.20.0), IRanges(v.2.18.1), S4Vectors(v.0.22.0), BiocGenerics(v.0.30.0), edgeR(v.3.26.5) and limma(v.3.40.2)
loaded via a namespace (and not attached): backports(v.1.1.5), fastmatch(v.1.1-0), aroma.light(v.3.14.0), plyr(v.1.8.4), lazyeval(v.0.2.2), splines(v.3.6.0), crosstalk(v.1.0.0), digest(v.0.6.22), htmltools(v.0.4.0), memoise(v.1.1.0), annotate(v.1.62.0), modelr(v.0.1.5), R.utils(v.2.9.0), prettyunits(v.1.0.2), colorspace(v.1.4-1), blob(v.1.2.0), rvest(v.0.3.5), rappdirs(v.0.3.1), haven(v.2.2.0), xfun(v.0.11), crayon(v.1.3.4), RCurl(v.1.95-4.12), jsonlite(v.1.6), genefilter(v.1.66.0), zeallot(v.0.1.0), zoo(v.1.8-7), survival(v.3.1-7), glue(v.1.3.1), gtable(v.0.3.0), zlibbioc(v.1.30.0), webshot(v.0.5.1), DESeq(v.1.36.0), DBI(v.1.0.0), viridisLite(v.0.3.0), xtable(v.1.8-4), progress(v.1.2.2), bit(v.1.1-14), truncnorm(v.1.0-8), htmlwidgets(v.1.5.1), httr(v.1.4.1), ellipsis(v.0.3.0), pkgconfig(v.2.0.3), XML(v.3.98-1.20), R.methodsS3(v.1.7.1), farver(v.2.0.1), locfit(v.1.5-9.1), tidyselect(v.0.2.5), labeling(v.0.3), rlang(v.0.4.1), later(v.1.0.0), AnnotationDbi(v.1.46.0), munsell(v.0.5.0), cellranger(v.1.1.0), tools(v.3.6.0), cli(v.1.1.0), generics(v.0.0.2), RSQLite(v.2.1.2), broom(v.0.5.2), ggdendro(v.0.1-20), evaluate(v.0.14), fastmap(v.1.0.1), yaml(v.2.2.0), bit64(v.0.9-7), nlme(v.3.1-142), mime(v.0.7), R.oo(v.1.23.0), xml2(v.1.2.2), compiler(v.3.6.0), rstudioapi(v.0.10), plotly(v.4.9.1), curl(v.4.2), interactiveDisplayBase(v.1.22.0), geneplotter(v.1.62.0), stringi(v.1.4.3), highr(v.0.8), GenomicFeatures(v.1.36.3), lattice(v.0.20-38), Matrix(v.1.2-17), vctrs(v.0.2.0), pillar(v.1.4.2), lifecycle(v.0.1.0), BiocManager(v.1.30.10), data.table(v.1.12.6), bitops(v.1.0-6), httpuv(v.1.5.2), R6(v.2.4.1), latticeExtra(v.0.6-28), hwriter(v.1.3.2), promises(v.1.1.0), gridExtra(v.2.3), MASS(v.7.3-51.4), assertthat(v.0.2.1), withr(v.2.1.2), GenomeInfoDbData(v.1.2.1), hms(v.0.5.2), rmarkdown(v.1.17), shiny(v.1.4.0) and lubridate(v.1.7.4)